Review



superscript ii rnase h2 reverse transcriptase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher superscript ii rnase h2 reverse transcriptase
    Superscript Ii Rnase H2 Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pm41316283-49-17-23
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h2 reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: SARS-CoV-2 pathogenesis in angiotensin II induced heart-on-a-chip disease model and exosome screening
    Article Snippet: Viral RNA (HCoV-229E, HCoV-NL63, SARS-CoV-2) was extracted from infected cell supernatant and Biowires separately using PureLink viral RNA/DNA extraction mini kit (Thermofisher Scientific). .. Following manufacturer protocol, viral RNA was reverse transcribed into cDNA using superscript II reverse transcriptase (Thermofisher Scientific). .. The amount of SARS- 4 CoV-2 RNA was measured by qPCR using SARS2 UpE F – ATTGTTGATGAGCCTGAAG and SARS2 UpE R – TTCGTACTCATCAGCTTG primers and PowerTrack SYBR Green Master Mix (Thermofisher scientific).

    Article Title: Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decay
    Article Snippet: Turbo DNA-free kit , ThermoFisher Scientific , AM1907. .. Superscript II Reverse Transcriptase , ThermoFisher Scientific , 18064014. .. Kapa HotStart PCR Kit , Roche , KAPA KK2502.

    Article Title: Recombinant high growth rate influenza virus comprising mutations in M1, NS2, and PB2
    Article Snippet: Briefly, the vRNA was used in a 40 ul reaction with 8 ul 5× E-PAP buffer, 4 ul 25 mM MnCl2, 2 ul 10 mM ATP, 1 ul RNAsin Plus (Promega), 1 ul E-PAP (polymerase) and 22 ul vRNA. .. The reaction was incubated at 37° C. for 1 hour. cDNA Synthesis Polyadenylated vRNA was used in the cDNA synthesis using Superscript II Reverse Transcriptase (Thermo Fisher Scientific). .. The 20 ul reverse transcriptase reaction was assembled as follows: 2 uL 5×FS Buffer, 1 ul TRSA Oligo (CGCAGTCGGTACTTTTTTTTTTTTTTTTTTVN, SEQ ID NO. 17), 1 ul TS oligo (AAGCAGTGGTATCAACGCAGAGTACGCrGrGrG, SEQ ID No. 18), 1 ul 10 mM dNTPs, 2 ul 0.1M DTT, 1 ul RNAsin Plus (Promega), 1 uL Superscript II enzyme and 9 ul polyadenylated vRNA.


    Article Title: Characterization of odor markers associated with aging in old ICR mice.
    Article Snippet: .. The total complementary DNA (cDNA) was synthesized against mRNA (4 μg) using 200 units of Superscript II Reverse Transcriptase (Thermo Scientific, Wilmington, DE, USA). .. The specific DNA fragments for each gene and β-actin were amplified from the mixture solution containing the cDNA template (1 μL), along with 2× Power SYBR Green (6 μL; Toyobo Life Science, Osaka, Japan) and specific primers for FMO3, NKA, AQP5 and FOXA1 genes (Supplementary Table S1) using qRT-PCR.

    Article Title: The GD3 ganglioside promotes cell growth, plasticity and chemotherapy resistance of human glioblastoma cancer stem cells.
    Article Snippet: Before use, RNA samples were treated with 1U ribonuclease-free deoxyribonuclease (Roche Applied Science, Meylan, France) at 37°C for 20 minutes. .. One μg of total RNA was used for reverse transcription using random hexamers and Superscript II reverse transcriptase as recommended by the manufacturer (ThermoFisher). ..

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Article Title: Repurposing host-guest chemistry to sequester virulence and eradicate biofilms in multidrug resistant Pseudomonas aeruginosa and Acinetobacter baumannii
    Article Snippet: .. The first strand cDNA was constructed using the SuperScript II Reverse Transcriptase (Thermo Fisher Scientific), while the second strand was constructed using random hexamers (Thermo Fisher Scientific) and HiFi HotStart Readymix (Kapa Biosystems). .. The sequencing library was prepared using the Nextera XT (Illumina) tagmentation reaction with 1 ng of RNA serving as an input, following manufacturer’s instructions.

    Incubation:

    Article Title: Recombinant high growth rate influenza virus comprising mutations in M1, NS2, and PB2
    Article Snippet: Briefly, the vRNA was used in a 40 ul reaction with 8 ul 5× E-PAP buffer, 4 ul 25 mM MnCl2, 2 ul 10 mM ATP, 1 ul RNAsin Plus (Promega), 1 ul E-PAP (polymerase) and 22 ul vRNA. .. The reaction was incubated at 37° C. for 1 hour. cDNA Synthesis Polyadenylated vRNA was used in the cDNA synthesis using Superscript II Reverse Transcriptase (Thermo Fisher Scientific). .. The 20 ul reverse transcriptase reaction was assembled as follows: 2 uL 5×FS Buffer, 1 ul TRSA Oligo (CGCAGTCGGTACTTTTTTTTTTTTTTTTTTVN, SEQ ID NO. 17), 1 ul TS oligo (AAGCAGTGGTATCAACGCAGAGTACGCrGrGrG, SEQ ID No. 18), 1 ul 10 mM dNTPs, 2 ul 0.1M DTT, 1 ul RNAsin Plus (Promega), 1 uL Superscript II enzyme and 9 ul polyadenylated vRNA.

    cDNA Synthesis:

    Article Title: Recombinant high growth rate influenza virus comprising mutations in M1, NS2, and PB2
    Article Snippet: Briefly, the vRNA was used in a 40 ul reaction with 8 ul 5× E-PAP buffer, 4 ul 25 mM MnCl2, 2 ul 10 mM ATP, 1 ul RNAsin Plus (Promega), 1 ul E-PAP (polymerase) and 22 ul vRNA. .. The reaction was incubated at 37° C. for 1 hour. cDNA Synthesis Polyadenylated vRNA was used in the cDNA synthesis using Superscript II Reverse Transcriptase (Thermo Fisher Scientific). .. The 20 ul reverse transcriptase reaction was assembled as follows: 2 uL 5×FS Buffer, 1 ul TRSA Oligo (CGCAGTCGGTACTTTTTTTTTTTTTTTTTTVN, SEQ ID NO. 17), 1 ul TS oligo (AAGCAGTGGTATCAACGCAGAGTACGCrGrGrG, SEQ ID No. 18), 1 ul 10 mM dNTPs, 2 ul 0.1M DTT, 1 ul RNAsin Plus (Promega), 1 uL Superscript II enzyme and 9 ul polyadenylated vRNA.

    Synthesized:

    Article Title: Characterization of odor markers associated with aging in old ICR mice.
    Article Snippet: .. The total complementary DNA (cDNA) was synthesized against mRNA (4 μg) using 200 units of Superscript II Reverse Transcriptase (Thermo Scientific, Wilmington, DE, USA). .. The specific DNA fragments for each gene and β-actin were amplified from the mixture solution containing the cDNA template (1 μL), along with 2× Power SYBR Green (6 μL; Toyobo Life Science, Osaka, Japan) and specific primers for FMO3, NKA, AQP5 and FOXA1 genes (Supplementary Table S1) using qRT-PCR.

    Amplification:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Purification:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Polymerase Chain Reaction:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Sequencing:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Construct:

    Article Title: Repurposing host-guest chemistry to sequester virulence and eradicate biofilms in multidrug resistant Pseudomonas aeruginosa and Acinetobacter baumannii
    Article Snippet: .. The first strand cDNA was constructed using the SuperScript II Reverse Transcriptase (Thermo Fisher Scientific), while the second strand was constructed using random hexamers (Thermo Fisher Scientific) and HiFi HotStart Readymix (Kapa Biosystems). .. The sequencing library was prepared using the Nextera XT (Illumina) tagmentation reaction with 1 ng of RNA serving as an input, following manufacturer’s instructions.



    Similar Products

    96
    TaKaRa superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/PrimeScript+II+Reverse+Transcriptase/pmc12664801-200-12-16
    Average 96 stars, based on 1 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    97
    New England Biolabs superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/ProtoScript+II+Reverse+Transcriptase/pm41106388-185-12-16
    Average 97 stars, based on 1 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    86
    Fisher Scientific superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/m+mlv+reverse+transcriptase/pmc12904577-326-5-9
    Average 86 stars, based on 1 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii rnase h2 reverse transcriptase
    Superscript Ii Rnase H2 Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pm41316283-49-17-23
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h2 reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii rnase h 2 reverse transcriptase
    Superscript Ii Rnase H 2 Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pmc12664216-50-17-24
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h 2 reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii rnase h reverse transcriptase kit
    Superscript Ii Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pm40930097-44-32-39
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h reverse transcriptase kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii rnase h2 reverse transcriptase kit
    Superscript Ii Rnase H2 Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pmc12530098-190-21-28
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h2 reverse transcriptase kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii rnase h2 reverse 24 transcriptase kit
    Superscript Ii Rnase H2 Reverse 24 Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pm41022577-194-29-37
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h2 reverse 24 transcriptase kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/high+capacity+cdna+reverse+transcription+kit/pmc12271857-83-25-29
    Average 90 stars, based on 1 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results